12 101 antibody  (Developmental Studies Hybridoma Bank)

Journal: Experimental gerontology

Article Title: Sustaining neuromuscular activation after total knee arthroplasty preserves skeletal muscle fiber size, contractility, and innervation in older adults

doi: 10.1016/j.exger.2025.112831

Figure Lengend Snippet: Effect of NMES on the response of muscle fiber size to total knee arthroplasty (TKA). Representative immunohistochemistry images for pre-surgery and 5-wk post-surgery Control ( A and B , respectively) and NMES ( C and D , respectively) groups. Tissue sections treated with antibodies recognizing myosin heavy chain (MHC) I (green), MHC IIA (red), and MHC IIX (blue) isoforms are shown. As fibers expressing only MHC IIX are rare, the large majority of MHC IIX expression occurs in MHC IIAX hybrid fibers (purple). Scale bar = 50 um. Skeletal muscle fiber cross-sectional area (CSA) calculated from minimal Feret diameter ( E ) for all fiber types pooled together (All Fibers) and MHC I, IIA, and IIAX fibers are shown at pre-surgery (open bar) and post-surgery (filled bar) for patients in control (black) and NMES (blue) groups. Analyses were limited to fibers expressing MHC I, IIA, and IIAX isoforms because other MHC isotype fibers (MHC I/IIA, IIX, and I/IIA/IIX) were too few to permit analyses. Bars represent mean and SE from parameter estimates from the linear mixed model analysis for n = 10 patients for each time point derived, except for n = 9 patients for MHC IIAX post-surgery because one patient did not have any MHC IIAX fibers at one time point. Data points represent individual volunteer average values for single fibers clustered within each volunteer for baseline and 5-week post-surgery evaluations. Please note that these individual average data are presented for data transparency purposes only and are not utilized in the statistical analytical model. Bar graphs represent least squared means and standard error values derived from the mixed model analysis. Deviation of individual values from bars graph data are due to the fact model-derived values reflect adjustment for the effects of sex, as well as other factors. In addition, many of the measures were log transformed for analysis, and as such the least squared means and standard errors plotted were back transformed into original units. Thus, these values can be somewhat offset from those of the raw data. P values indicate time (T) and group X time (GxT) effects (atop brackets) or within group comparisons over time (over lines). * P < 0.05, *** P < 0.001. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: Briefly, primary antibodies raised against the three main skeletal muscle MHC isoforms (MHC I, IIA, IIX; BA-D5, SC-71, and 6H-1 from Developmental Studies Hybridoma Bank, Iowa City, IA) are used to identify all 6 fiber isotypes (MHC I, I/IIA, IIA, IIA/X, IIX, I/IIA/IIX).

Techniques: Immunohistochemistry, Control, Expressing, Derivative Assay, Transformation Assay

Journal: Frontiers in Cell and Developmental Biology

Article Title: Enlargement of the muscle stem cell pool in linc-MYH-deficient mice does not prevent sarcopenia during aging

doi: 10.3389/fcell.2025.1667437

Figure Lengend Snippet: Enlargement of the MuSC pool does not attenuate sarcopenia during aging. (a) MRI cross-sectional images illustrating distribution of fat (cyan) and skeletal muscles (brown) in aging mice. Only the lower body was included for the statistical analysis. The bar graphs show mean volumes of muscle tissues at 15, 18, and 24 months (15-month: n = 13 WT, n = 17 linc-MYH −/− , 18-month: n = 13 WT, n = 15 linc-MYH −/− , 24-month: n = 5 WT, n = 8 linc-MYH −/− ), ** p < 0.01, *** p < 0.001. (b) Body weights of WT and linc-MYH −/− mice at 15, 18, and 24 months (15-month: n = 13 WT, n = 17 linc-MYH −/− ; 18-month: n = 13 WT, n = 15 linc-MYH −/− ; 24-month: n = 6 WT, n = 8 linc-MYH −/− ). (c) Muscle weights of tibialis anterior (TA), extensor digitorum longus (EDL), and soleus muscles at 24 months (n = 10 WT, n = 8 linc-MYH −/− ). (d,e) Cross-sectional areas of different fiber types in TA or soleus muscles at 10 weeks (young) and 24 months (aging). Data for type II fibers were obtained from TA muscle (10-week: n = 3 WT, n = 2 linc-MYH −/− ; 24-month: n = 4 WT, n = 3 linc-MYH −/− ), whereas data for type I fibers were obtained from soleus muscles (10-week: n = 3 WT, n = 3 linc-MYH −/− ; 24-month: n = 3 WT, n = 3 linc-MYH −/− ).

Article Snippet: Identification of skeletal muscle fiber types was performed by a combination of antibodies against MyHC type I (BA-D5, DSHB), MyHC type IIA (SC-71, DSHB), and MyHC type IIB (BF-F3, DSHB).

Techniques: Muscles

Journal: Frontiers in Cell and Developmental Biology

Article Title: Enlargement of the muscle stem cell pool in linc-MYH-deficient mice does not prevent sarcopenia during aging

doi: 10.3389/fcell.2025.1667437

Figure Lengend Snippet: Inactivation of linc-MYH increases the number of slow-twitch muscle fibers in soleus muscles of geriatric mice. (a) Representative images of muscle fibers in TA and soleus muscles stained with antibodies against different MyHC isoforms. (b) Stacked bar graph showing the proportion of type I, type IIa, type IIb and type IIx fibers in cross-sectioned TA and soleus muscles (n = 4 WT, n = 4 linc-MYH −/− ), ** p < 0.01, **** p < 0.0001. (c) Schematic representation of different muscle fiber types and metabolic characteristics. Inactivation of linc-MYH induces a shift toward oxidative muscle fibers.

Article Snippet: Identification of skeletal muscle fiber types was performed by a combination of antibodies against MyHC type I (BA-D5, DSHB), MyHC type IIA (SC-71, DSHB), and MyHC type IIB (BF-F3, DSHB).

Techniques: Muscles, Staining